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1. The DNA template is that particular DNA sequence which you want copied. · 2. There are two requirements for a suitable DNA polymerase enzyme for PCR. · 3.Reaction set-up for other primer sets will require a different spreadsheet. To set up the above PCR reaction, proceed as follows: 1. Wear gloves. 2. Remove components and parameters involved in real-time PCR experiments. A more detailed discussion of PCR results from samples containing different amounts of. PCR Setup—Six Critical Components to Consider · Template DNA · DNA polymerase · Primers · Deoxynucleoside triphosphates (dNTPs) · Magnesium ion (Mg2+) · Buffer. The polymerase chain reaction (PCR) was developed by. Kerry Mullis who won the Nobel Prize for this work. PCR can be used for gene cloning and manipulation, The following components are needed to perform PCR in the laboratory: 1) DNA (DNA of interest that contains the target sequence you wish to copy).