Intravital Confocal and Two-Photon Imaging of Dual-Color Cells and Extracellular Matrix Mimics Published online by Cambridge University Press: 04 February 2013 Ufuk Bal , Volker Andresen , Brenda Baggett and Urs Utzinger Article Figures Metrics Rights & Permissions Abstract Fig. S9. Intravital imaging of intestinal stem cells through the AIW. Fig. S10. Intravital imaging of pancreatic tissue through the AIW. Fig. S11. Intravital imaging of OVA-reacting T cells in the spleen through the AIW. Movies S1 to S4 legends Other Supplementary Material for this manuscript includes the following: (available at Long-term time-lapse multimodal intravital imaging of regeneration and bone-marrow-derived cell dynamics in skin Benedikt W. Graf1,2, Eric J. Chaney1, Marina Marjanovic1, Steven G. Adie1, Michael De Lisio1,3, M. Carmen Valero1, Marni D. Boppart1,3 & Stephen A. Boppart1,2,4 A major challenge for translating cell-based therapies is understanding the dynamics of cells and cell populations in Intravital molecular imaging data indicated that tumor cell death usually contributes to the accumulated effect of multiple CTLs in the tumor lesions. Compared with a normal immune organ (spleen) the capacity of CTLs to kill tumor cells is restrained in the liver, with one CTL killing only 1.24 ± 0.11 tumor cells per day. Intravital imaging (IVI) enables the visualization and analysis of tumour cell dynamics in live animals and in real time. Previous research has used IVI to observe benign and malignant diseases of Download PDF. Download PDF. Methodology article; Open Access; Published: Condeelis J, Segall JE: Intravital imaging of cell movement in tumours. Nat Rev Cancer. 2003, 3 (12): 921-930. 10.1038/nrc1231. Additional File 1: The fluorescent cells observed after DNA tattooing are predominately keratinocytes. This section describes that the Mosaic time lapse and z-series intravital imaging in transgenic mice allows acquisition of very large volumes of tumor heterogeneity that can be zoomed to follow single cell phenotypes in real time. A-C) Top panels: A single z plane showing large area imaging of a mammary tumor within a transgenic PyMT mouse expressing fluorescent proteins. Intravital microscopy is a form of microscopy that allows observing biological processes in live animals ( in vivo) at a high resolution that makes distinguishing between individual cells of a tissue possible. [1] In mammals, in some experimental settings a surgical implantation of an imaging window is performed prior to intravital microscopy. Intravital imaging of tumors in live rodents has been used suc - associated macrophages (i.e., single file of unattached tumor cells and alternating macrophages following one another). This type of distinct patterns of cell movement were consistently observed: streaming movement, defined as coordinated cell motility whereby High-resolution intravital imaging (IVM) has proven to be a powerful technique to visualise dynamic processes that are important for tumour progression, such as the interplay between tumour cells and cellular components of the tumour microenvironment. The development of IVM tools, including imaging windows and photo-marking of individual cells, has led to the visualisation of dynamic processes This experiment demonstrates the use of intravital imaging